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The biological activities of twelve different kinds of enzymatic browning reaction products(EBRP), which resulted from the reactants tour kinds of polyphenols with polyphenol oxidase extracted from Ligularia jtschen; pimpinella brachycarpa and Aster scaber of edible mountain herbs. All of twelve samples did not show any mutagenic effect in the spore rec-assay, Ames mutagenicity test and DNA breaking test. However metal ions such as Cu^(2+), Fe^(2+), and Ni^(2-) were increased the DNA breakage in rec-assay. The EBRPs inhibited the mutagenicities induced by benzo(α)pyrene(B(α)P),3-amino-1, 4dimethyl-SH-pyrido-[4, 3-b]indole(Trp-P-1) and 2-aminofluorene(2-AF) in Salmonella/microsome assay system with S-9 mix. In effects of EBRPs on the DNA repair system, the activity of EcoRI was highly inhibited and that of T, DNA ligase was inactivated by addition of EBRPs. The results of transformation ratio of plasmid pGA658 into E. coli HB 101 was significantly decreased by the reaction products of S. brachycarpa polyphenoloxidase(PPO). When I1V light was exposed to the mixture of DNA and EBRP before the thanformation, the reaction products from L. fischeri PPO with pyrogallol, catechol and hydroxyhydroquinone stimulated transformation ratio.
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